Some Applications of High Performance Liquied Chromatography -Determination of Trimethoprim

A simple, fast and precise high performance liquid chromatographic method for the assay of trimethoprim in the presence of sulphamethoxazole has been developed. The procedure is based on using acetonitril: water: acetic acid in the ratio of 30:69.9:0.1 (v : v : v) as a mobile phase, flow rate (1ml/min.) and uv detector (254nm.).Each analysis required no longer than 10 minutes. the detector responses were linear in the range of 0.05-120μg/ml for trimethoprim with a relative standard deviation of 0.49-0.971% and a relative error of 1-2.5% . The proposed method was also applied satisfactorily to the pharmaceutical preparation.